Abstract #1406834: Study of Methylation Status and Expression of Vitamin D Receptor Gene in Polycystic Ovarian Syndrome Patients of Kashmiri Population

Downregulation of Vitamin D Receptor (VDR) gene expression resulting from epigenetics events, like methylation in promoter VDR play an important role pathogenesis, clinical and bio-chemical parameters polycystic ovary syndrome, but the results different studies are inconsistent. We aim to determine status analysis addition co-relation between profile genes PCOS patients Kashmiri Population. This study was a case control, comprising 100 women with normal healthy control aged 15-45 years. were selected after proper diagnosis using Rotterdam criteria (2004) obtaining written consent. DNA extraction carried out by non-enzymatic Salting method extracted samples subjected bisulphite treatment EZ Methylation-Gold Kit (Zymo Research, Irvine, California) produces chemical conversion unmethylated cytosine uracil, which is detected as thymine PCR. Methylation specific PCR (MS-PCR) performed following bisulfite conversion, pre-designed sets methylated primers confirm prominence region specially acknowledge fragments. The RNA blood sample processed ahead for cDNA synthesis reverse transcriptase reaction then relative RT-PCR SYBR Green fluorescent dye. found that vitamin level among test group significantly lower compared controls (13.95±9.60 VS. 16.63±7.46; p=0.026). higher (84% vs 62%; p<0.0001) OR 3.21. Furthermore, there exists significant difference average cyclic threshold (CT) value groups (24.64 vs. 26.14;p <0.0001). CT corresponding GAPDH (glyceraldehyde phosphate dehydrogenase) 29.76 32.11 group. 0.55 times down regulated comparison controls. In present study, we have comprehensively shown hypermethylation associated reduced decreased women. Increased response levels could be involved impairment mediated effects peculiar derangements syndrome. However, enhanced ligand availability receptor maintain homeostasis signalling. Hence, this, inverse association altered thereby effecting cell patients.